Anna Moorhouse

At the age of 11, Moorhouse joined the Chadderton Grasshoppers boys' team where she spent three years before no longer being allowed to play on a boys' team. She switched to Chaddy End girls team, and also represented Oldham Athletic. In 2011, age 16, Moorhouse was scouted by Manchester United and spent a year with the team's Regional Talent Club. It was also at 16 that she began playing in goal.

Everton

Due to Manchester United's lack of a senior women's team, Moorhouse moved to Everton to begin her senior career in 2012. She did not make an appearance for Everton in her two seasons at the club, ranking behind Rachel Brown and Danielle Hill, with Lizzie Durack and Megan Walsh joining in her second season.

Durham

In 2014, Moorhouse joined newly formed WSL 2 club Durham. She spent two seasons with the club, making a total of 15 appearances.

Doncaster Rovers Belles

In 2016, Moorhouse made the jump up to the WSL 1 with Doncaster Rovers Belles. She made four appearances including on the last day of the 2016 season when already-relegated Doncaster, having lost all 15 of their league games, beat Reading 1–0.

Arsenal

Ahead of the 2017 FA WSL Spring Series, Moorhouse signed with Arsenal. She made her debut on 20 May 2017, in the final game of the Spring Series, a 4–2 victory over Birmingham City. During the 2017–18 season, she made four league appearances behind Sari van Veenendaal but was used more consistently in the early stages of both the FA Cup and League Cup. Arsenal reached the final of both competitions, with Moorhouse an unused substitute. Arsenal won the League Cup but lost the FA Cup.

West Ham United

In July 2018, Moorhouse left Arsenal to join newly promoted FA WSL team West Ham United. She split playing time with Rebecca Spencer, each making 10 league starts. Moorhouse was named the starter in all five FA Cup…

In July 2024, Moorhouse received her first senior international call-up for England for Euro 2025 qualifying matches against the Republic of Ireland and Sweden. She did not feature in either matchday squad. Moorhouse was recalled to the squad for the following international window, this time as an unused substitute for friendlies against Germany and South Africa. On 5 June 2025, Moorhouse was named in England's squad for UEFA Euro 2025. She was an unused substitute as England successfully defended their European title. On 29 November 2025, Moorhouse made her senior international debut in an 8–0 friendly victory against China. Following her debut, Moorhouse was awarded legacy number 239.

Moorhouse was born in Oldham, Greater Manchester. She attended Hulme Grammar School.